1.
Z Geburtshilfe Neonatol
; 227(2): 141-146, 2023 Apr.
Artículo
en Alemán
| MEDLINE
| ID: mdl-36368684
2.
FEBS Lett
; 581(18): 3396-404, 2007 Jul 24.
Artículo
en Inglés
| MEDLINE
| ID: mdl-17618624
RESUMEN
Autophagy depends on the activity of phosphoinositide-3 kinase class III to generate PI(3)P. We identified the human WIPI protein family of PI(3)P-binding factors and showed that WIPI-1 (Atg18) is linked to autophagy in human cells. Induction of autophagy by rapamycin, gleevec, thapsigargin and amino acid deprivation led to an accumulation of WIPI-1 at LC3-positive membrane structures (WIPI-1 puncta-formation), suggested to represent autophagosomal isolation membranes. WIPI-1 puncta-formation is inhibited by wortmannin and LY294002, and PI(3)P-binding-deficient WIPI-1 is puncta-formation-incompetent. Quantification of WIPI-1 puncta should be suitable to assay mammalian autophagy.